Alignment of Cancer borealis and Homarus americanus glycoprotein hormone and trissin precursor proteins. (A1) MAFFT alignment of the putative C. borealis and H. americanus glycoprotein hormone α2 subunit precursors (Canbo-pre-GPα2; deduced from GEFB01019143; Homam-pre-GPα2; deduced from GFDA01013881). (A2) MAFFT alignment of the putative C. borealis and H. americanus glycoprotein hormone β5 subunit precursors (Canbo-pre-GPβ5; deduced from GEFB01005773; Homam-pre-GPβ5; deduced from GFDA01059529). (B) MAFFT alignment of the putative C. borealis and H. americanus trissin precursors (Canbo-prepro-Tris; deduced from GEFB01013178; Homam-prepro-Tris; deduced from GFDA01095285). In the line immediately below each sequence grouping, “*” indicates identical amino acid residues, while “:” and “” denote amino acids that are similar in structure between sequences. In this figure, signal peptides are shown in gray, while all mono/dibasic cleavage loci are shown in black. For each sequence, the isoform of the peptide for which the precursor is named is shown in red; in B, trissin linker/precursor related peptides shown in blue.