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. 2020 Mar 18;15(3):e0229290. doi: 10.1371/journal.pone.0229290

Fig 3. Exercise reduces variance in perfusion in EO771 tumours.

Fig 3

(a) Representative immunofluorescent images of sections containing Hoechst 33342 (perfused blood vessels, blue) and stained for pimonidazole (hypoxia, green) in B16-F10 and EO771 tumours from non-exercising vs exercising mice. Quantification of hypoxic area in B16-F10 (b) and EO771 (d) tumours from non-exercising vs exercising mice. Quantification of perfused blood vessels in B16-F10 (c) and EO771 (e) tumours from non-exercising vs exercising mice. B16-F10 No Ex and Ex: n = 12, EO771 No Ex: n = 9 (two mice had intra-peritoneal tumours and one tumour exhibited low-level, diffuse perfusion which could not be quantified) and Ex: n = 10 (one mouse had intra-peritoneal tumours, and one was euthanised before tumour development due to pyometra). Difference in variance analysed using the F test. Data are presented as individual data points and mean ± 95% CI.