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. 2020 Mar 18;16(3):e1008335. doi: 10.1371/journal.ppat.1008335

Fig 3. HSV-1 infection induces IL-1β expression and secretion.

Fig 3

(A–K) TPA-differentiated THP-1 macrophages were treated with 2 μM Nigericin for 2 h, and infected with HSV-1 at MOI = 0.1, 0.2, 0.4 and 0.8 for 8 h (A, C, E and G), infected with HSV-1 at MOI = 0.8 for 2, 4, 6 and 8 h (B, D, F and H), or transfected with HSV120 (3 μg/ml) for 2, 4 and 6 h (I–K). (L–O) Primary MEFs were primed with LPS (1 μg/ml) for 6 h, and then treated with 5 mM ATP for 2 h or infected with HSV-1 (MOI = 1) (L and N) or transfected with HSV120 (3 μg/ml) (M, O) for 2, 4 and 6 h. (A, B, I, L and M) IL-1β protein was determined by ELISA. (C, D and J) Matured IL-1β (p17) and cleaved Casp-1 in supernatants (top) and pro-IL-1β production in lysates (bottom) were determined by Western-blot analyses. (E, F, G, H, K, N and O) IL-1β mRNA and GAPDH mRNA (E, F), HSV-1 ICP27 mRNA and GAPDH mRNA (G, H and N), and IFN-β mRNA and GAPDH mRNA (K, O) were quantified by RT-PCR. Data shown are means ± SEMs; *p < 0.05, **p < 0.01, ***p < 0.0001; ns, no significance.