Skip to main content
. 2020 Mar 6;16(3):e1008524. doi: 10.1371/journal.pgen.1008524

Fig 5. RNF2-deficient cells depend on the Fanconi Anemia fork-protective proteins for genome maintenance and survival.

Fig 5

A. (Top) Representative images of EdU and γH2AX foci in WT and RNF2 KO T80 cells, where indicated FANCD2 and FANCI were also depleted by siRNA. (Bottom left) Quantification of the γH2AX RFI in EdU positive cells (N = 75 from 3 biological replicates). (Bottom right) Verification of knockdown efficiency by western blot. B. (Top) Representative images showing the PLA signal between γH2AX and EdU-labeled replication forks is enhanced by the co-knockdown of RNF2 with either FANCD2 or FANCI in U2OS cells. siC indicates scrambled control siRNAs. (Bottom) Quantification of the number of PLA signals per nucleus under the indicated conditions (N = 50 from 3 biological replicates). C. Viability of the T80 RNF2 KO cells is decreased by the depletion of FANCD2 or FANCI by siRNA (N = 6 biological replicates). D. Model for our findings.