. 2020 Mar 18;15(3):e0230496. doi: 10.1371/journal.pone.0230496

Table 1. Primers and probes used in this study.

Application	Species	Oligonucleotide sequence	Target, size (bp)
TaqMan	Bison priscus	F, `CCCCAGCAAATCCACTCAATACA`	CYTB, 81
		R, `TTGATCGTAAAATTGCGTATGCAAATAAG`
		Probe, `CCTCCCCACATCAAAC`
TaqMan	Bison schoetensacki	F, `CTACTAGTACTATTCGCACCCGA`	CYTB, 75
		R, `AGGCGTGTTAAGTGGATTTGCT`
		Probe, `CCTCCTCGGAGACCCAG`
TaqMan	Bos primigenius	F, `CCAGCCAATCCACTCAACACA`	CYTB, 79
		R, `TTGATCGTAAGATTGCGTATGCAAA`
		Probe, `CCCCTCACATCAAACC`
TaqMan	Rangifer tarandus	F, `TCACATCTGTCGAGACGTCAATT`	CYTB, 61
		R, `TGCTCCGTTGGCATGTATGTA`
		Probe, `TGGCTGAATCATCCG`
TaqMan	Cervus elaphus	F, `CATGTAGGGCGAGGCCTGTA`	CYTB, 66
		R, `AACTACTCCGATGTTTCACGTCTCT`
		Probe, `ACGGATCATATACTTTTC`
TaqMan	Crocuta crocuta	F, `TGGCGAGACATTATCCGAGAA`	COX3, 68
		R, `CGCAGCCCCTTTTGTACAGT`
		Probe, `CACATTCCAGGGACACC`
PCR-sequencing	Bison priscus	F, `CCAAACCCTTAAACTATCCCTC`	ND5, 74
PCR-sequencing	Bison priscus	R, `CGAATAGTGCTACTGGGACA`	ND5, 74
PCR-sequencing	Bison schoetensacki	F, `CTACTAGTACTATTCGCACCCGA`	CYTB, 75
PCR-sequencing	Bison schoetensacki	R, `AGGCGTGTTAAGTGGATTTGCT`	CYTB, 75
PCR-sequencing	Rangifer tarandus	F, `ATCCGATACAATAACAGCAT`	CYTB, 65
PCR-sequencing	Rangifer tarandus	R, `TTCAGCCATAATTGACGTCT`	CYTB, 65

Oligonucleotides sequences are displayed in the 5’-3’ orientation. F, forward PCR primer; R, reverse PCR primer; Probe, TaqMan minor groove binder (MGB) probe labeled with 6-carboxyfluorescein (FAM). The last column indicates the region of the mitochondrial genome amplified with each primer set and the size of the amplicon.