Fig. 2.

Establishment of primary osteocyte cultures from GHRKO and control mice. Long bones (femur, tibia, and humerus) were used to establish primary osteocyte cultures. The purity of each preparation was verified by expression of osteocyte-specific genes using real-time polymerase chain reaction. RNA from differentiated osteocyte-like IDG-SW3 cell line was used as a reference. Shown is a representative experiment with primary osteocyte cultures derived from 2-month-old control (n = 4) and GHRKO male mice (n = 6). Data are presented as mean ± SEM, and significance was accepted at p < 0.05.