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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: J Bone Miner Res. 2018 Sep 14;34(1):106–122. doi: 10.1002/jbmr.3573

Fig. 5.

Fig. 5.

NADH redox state in primary osteocyte cultures from control and GHRKO mice. Cells were seeded (0.4 × 106 cells/mL) on collagen-coated glass plates and visualized using the 2000E Nikon Microscope Eclipse TE microscope at 20× magnification. (A) Schematic representation of redox index calculations: basal level relative to maximal respiration after carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP; 1 μM) (0%) and minimal respiration after NaCN (1 mM) (100%). (B) Time lapse of NADH autofluorescence in osteocytes from male mice, and (C) NADH redox index in osteocytes from male mice calculated according to (A). (D) Time lapse of NADH autofluorescence in osteocytes from female mice, and (E) NADH redox index in osteocytes from female mice calculated according to (A). Data are presented as mean ± SEM of 20 cells per time point.