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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: J Bone Miner Res. 2018 Sep 14;34(1):106–122. doi: 10.1002/jbmr.3573

Fig. 8.

Fig. 8.

Osteocyte response to glucose deprivation. Primary osteocytes from male mice were seeded (0.4 × 106 cells/mL) on collagen-coated plates for 10 to 15 days. (A) Expression levels of GLUT-1 were determined by real-time PCR (control n = 10 samples from >20 mice; GHRKO n = 5 samples from 12 mice). (B) GLUT-1 and GLUT-4 protein levels were determined from cell lysates of primary osteocytes by Western immunoblotting (control n = 8 samples from >12 mice; GHRKO n = 6 samples from 10 mice). (C) Total and phosphorylated AMPKa and b were determined from cell lysates of primary osteocytes with or without (4 to 6 hours) glucose by Western immunoblotting (control n = 4 mice; GHRKO n = 4 mice). (D) LC3II and cleaved caspase 3 (CC3) were determined from cell lysates of primary osteocytes by Western immunoblotting (control n = 4 mice; GHRKO n = 4 mice). Data are presented as mean ± SEM of multiple assays. Significance was accepted at p < 0.05.