Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HTLA cells (HEKT based) | PMID:25895059 | ||
| Transfected construct (Homo sapiens) | MTNR1A | PMID:25895059 | AddGene #66443 | |
| Recombinant DNA reagent, PCR primers | MTNR1A N162Q Forward |
This paper | CTGCCGTCCTGCCGcaaCTGAGGGCAGGCAC | |
| Recombinant DNA reagent, PCR primers | MTNR1A N162Q Reverse | This paper | GTGCCTGCCCTCAGttgCGGCAGGACGGCAG | |
| Recombinant DNA reagent, PCR primers | MTNR1A Y281F Forward | This paper | GTTCGTAGCGAGCTtCTACATGGCTTAC | |
| Recombinant DNA reagent, PCR primers | MTNR1A Y281F Reverse | This paper | GTAAGCCATGTAGaAGCTCGCTACGAAC | |
| Commercial assay or kit | BrightGlo Reagent | Promega.com | Cat # E2610 | |
| Chemical compound, drug | Hit compounds | Enamine, Molport, Chembridge |
See 62 compounds listed in Supplementary file 1 | |
| Software, algorithm | ICM-Pro, V3.8–7 |
Molsof.com | ||
| cell line (Homo sapiens) | HEK293 T | ATCC | CRL-11268 | |
| transfected construct (Homo sapiens) | Human MT1 | PMID:31019306 | ||
| Chemical compound, drug | Luciferin | Goldbio.com | Cat#: LUCNA-1G |