Figure 4. Loss of Rictor reduces evoked SV release efficiency but Raptor loss does not.

(A) Plot showing no difference in the vesicular release probability of Raptor-Con (black) and Raptor-KO (red) neurons. (B₁) Example traces showing the rate of SV release (top traces) over their corresponding EPSCs from Raptor-Con (black) and Raptor-KO (red) neurons. (B₂) Plot of the peak vesicle release rates of Raptor-Con (black) and Raptor-KO (red) neurons. (C₁) Example traces of EPSCs evoked in response to 2 ms depolarizations spaced at 25, 50, and 100 ms. The three sweeps at different intervals are overlayed, as are the responses from Raptor-Con (black) and Raptor-KO (red) neurons. The values are normalized to the peak amplitude of the first EPSC in each sweep. (C₂) Summary data showing the estimated marginal means and standard errors for Raptor-Con (black) and Raptor-KO (red) groups at different interpulse intervals. All data summarized in panels (A-C) were obtained from three independent cultures. (D) Plot showing the decrease in vesicular release probability between Rictor-Con (black) and Rictor-KO (blue) neurons. (E₁) Example traces showing the rate of SV release (top traces) over their corresponding EPSCs from Rictor-Con (black) and Rictor-KO (blue) neurons. (E₂) Plot of the peak vesicle release rates of Raptor-Con (black) and Raptor-KO (red) neurons. (F₁) Example traces of EPSCs evoked in response to 2 ms depolarizations spaced at 25, 50, and 100 ms. The three sweeps at different intervals are overlayed, as are the responses from Rictor-Con (black) and Rictor-KO (blue) neurons. The values are normalized to the peak amplitude of the first EPSC in each sweep. (F₂) Summary data showing the estimated marginal means and standard errors for Rictor-Con (black) and Raptor-KO (red) groups at different interpulse intervals. All data in panels D-F were obtained from four independent cultures. * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001, and n.s. = p > 0.05, effect of group tested with Generalized Estimating Equations.