Skip to main content
. 2020 Mar 18;6(12):eaaz3367. doi: 10.1126/sciadv.aaz3367

Table 1. List of mutagenic oligonucleotide primers.

Primer name Primer sequence
Region1-f 5′-ggctggccccattgtgtttttcttattattattgtagacaagagagtatagattaa-3′
Region1-r 5′-ttaatctatactctcttgtctacaataataataagaaaaacacaatggggccagcc-3′
Region2-f 5′-gtaggatggaatgcatcccaattattccgatcagcaaaatcatgaa-3′
Region2-r 5′-ttcatgattttgctgatcggaataattgggatgcattccatcctac-3′
Region3-f 5′-aaaaaagagacgaagataacttagaacattattatttgcaaaaagcccttctgccaccagtgtagg-3′
Region3-r 5′-cctacactggtggcagaagggctttttgcaaataataatgttctaagttatcttcgtctctttttt-3′
Region4-f 5′-taacatctgtcccattgaaatctgtaatggattattgatagagctggttgtatacataaaaaagagacg-3′
Region4-r 5′-cgtctcttttttatgtatacaaccagctctatcaataatccattacagatttcaatgggacagatgtta-3′
Region5-f 5′-ctcccacaaaggactgcagttcttcattattgctaaatcttgtgacaaagattgcc-3′
Region5-r 5′-ggcaatctttgtcacaagatttagcaataatgaagaactgcagtcctttgtgggag-3′