PEM‐seq |
Detection of off‐target effects. Simultaneously determines the editing efficiency and specificity of CRISPR/Cas9 |
– |
46
|
CRISPR‐PLANT v2 |
CRISPRPLANT v2 detects every off‐target |
https://www.genome.arizona.edu/crispr2/
|
83
|
CCTop |
Employs position‐dependent weight coefficients in their off‐target scoring algorithms |
https://crispr.cos.uni-heidelberg.de
|
79
|
CROP‐IT |
Scoring potential off‐target sites by the division of protospacer into three segments with weight coefficients optimized/rained with ChIP‐Seq data |
https://www.adlilab.org/CROP-IT/homepage.html
|
85
|
CHOPCHOP |
Rapid and easy selection of the optimal CRISPR/Cas9 or TALEN target sequences in genes from various organisms |
https://chopchop.cbu.uib.no/, https://chopchop.rc.fasharvard.edu
|
86
|
CHOPCHOP v2 |
Web‐based tool for GE based on TALEN and CRISPR. A powerful and intuitive tool that serves both beginners and experienced users |
https://chopchop.cbu.uib.no
|
138
|
CFD score |
A scoring tool for a mismatch position and sequence‐dependent off‐target |
https://www.broadinstitute.org/rnai/public/analysis-tools/sgrna-design
https://research.microsoft.com/en-us/projects/azimuth/
|
78
|
Feng Zhang lab's Target Finder |
Employs position‐dependent weight coefficients in their off‐target scoring algorithms |
https://crispr.mit.edu/
|
18, 38
|
CT‐Finder |
Predicts genomic off‐target sites |
https://bioinfolab.miamioh.edu/ct-finder
|
87
|
CRISPOR |
A web‐based tool which finds gRNAs in an input sequence and ranks them according to different scores evaluating potential off‐targets in the genome and predicts on‐target activity |
https://crispor.org
|
44
|
CRISPR‐GE |
Expedites experimental design and analyzes mutations for genome editing based on Cpf1/CRISPR/Cas9 in various organisms including plants |
https://skl.scau.edu.cn/
|
89
|
An ensemble learning method |
Predicts the off‐target sites of sgRNAs |
https://github.com/penn‐hui/OfftargetPredict
|
48
|
Cas‐OFFinder |
Searches the potential off‐target sites in user‐defined sequences or given genome |
https://www.rgenome.net/cas-offinder/
|
77
|
MD |
Analysis of the molecular function of CRISPR/Cas9 |
– |
139
|