Table 3.

Tools to design target‐sgRNA

Tools	Description	Web source	Ref.
CRISPR‐P 2.0	Web service for computer‐aided designing of sgRNA with minimal chances of off‐target potentials	https://cbi.hzau.edu.cn/CRISPR2/	91
E‐CRISP	Web‐based application for designing gRNA sequences. Provides experiment‐oriented design and flexible output parameters that enables the design of many libraries	https://www.e-crisp.org/, https://www.e-crisp.org/E-CRISP/	92
Breaking‐Cas system	Web service for designing gRNAs for CRISPR/Cas9 system and other newly generated CRISPR/Cas systems available via ENSEMBL	https://bioinfogp.cnb.csic.es/tools/breakingcas	93
CasFinder	Extends and modifies a method to search the potential off‐targets for Cas9 by using queries that combine PAMs and seeds	https://arep.med.harvard.edu/CasFinder	18
CRISPR Design Tool	A computational tool, facilitating the selection and validation of sgRNAs and prediction of off‐target	https://www.genome-engineering.org/	38