Figure 6.

Association of capsules with lung cell populations in mice. (Fe^III‐TA)₁, (Fe^III‐TA)₃, and (Fe^III‐TA)₆ capsules (1 × 10⁷ capsules in 100 µL DPBS) were administered intratracheally. Following administration at 20 h, the mice were euthanized and cell suspensions were prepared from lungs for analysis by mass cytometry. a) Identification of free and cell‐associated capsules in lung digests by mass cytometry. Cell‐associated capsules were identified based on high DNA staining. b) Relative proportions of free and cell‐associated capsules in lung digests. Bars show mean values (n = 4). c) t‐Stochastic neighbor embedding (tSNE) plot from pooled lung samples showing gating of cell populations of interest. d) Representative tSNE overlay plots of mice that were administered with the indicated capsule type; red and blue dots are cells with and without capsule‐associated signals, respectively. e) Percentage of cell population showing capsule association. f) Median number of capsules per cell for the indicated cell populations. e,f) Data are shown as the mean ± SD (n = 4). * indicates p < 0.05 versus all other cell populations; (*) indicates significant versus endothelial cells, B cells, T cells, alveolar macrophages, and Ly6G^int neutrophils; and ^# indicates p < 0.05 between the capsule groups indicated (one‐way ANOVA with Tukey's multiple comparisons test).