Table 1.
Ionization mode and selected reaction-monitoring ion pairs for the detection of curcumin, d6-curcumin, and their conjugates in plasma samples.
| Aglycon/conjugate | Ionization mode | Molecular ion(m/z) | Fragment ion(m/z) | Molecular ion(m/z) | Fragment ion(m/z) |
|---|---|---|---|---|---|
| curcumin | d6-curcumin | ||||
| Curcumina | pos. | 369.0 | 177.0 | 375.25 | 180.24 |
| C-Gb | neg. | 543.48 | 216.99 | 549.48 | 220.42 |
| C-Sa | pos. | 449.38 | 285.38 | 455.38 | 291.0 |
| C-diGb | pos. | 721.60 | 369.20 | 727.60 | 375.18 |
| C-diSa | pos. | 529.51 | 449.38 | 535.50 | 375.18 |
| C-SGa | neg. | 623.60 | 216.99 | 629.60 | 220.42 |
| C-SdiGb | pos. | 801.70 | 369.20 | 807.70 | 375.18 |
C, curcumin; G, glucuronide; S, sulfate; pos., positive; neg., negative.
Paper strips were eluted with a H2O/acetonitrile (50:50) or b H2O/acetonitrile (20:80).