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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Exp Mol Pathol. 2020 Jan 7;113:104369. doi: 10.1016/j.yexmp.2020.104369

Fig. 9. Oval cells in NASH (n=3 tissue replicates).

Fig. 9.

(A) Steatosis with inflammatory cells and additional hepatic epithelial cells (left side, upper left panel). Oval cells were prevalent (top middle and right panels; arrows, inset of boxed areas). Middle panels show Cx43 and albumin costaining. Bile duct cells expressed Cx43 (left), hepatocytes expressed albumin (middle) and oval cells expressed Cx43 with or without albumin (right). In oval cells, γH2AX or p21 were absent but Ki67 was widely expressed (red color; arrows) (bottom panels). This contrasted with hepatocytes. Nuclei stained byHoechst or DAPI (blue); original magnification x 100-400, scale bar, 10μm. (B) Events in oval cells vs adjacent hepatocytes by morphometry showed major differences, p<0.05. (C) Ploidy in oval cells revealed 2Nor 4N and no8N+. Cells with aneuploid DNA were distributed across ploidy classes.