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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 24;459(2):72–78. doi: 10.1016/j.ydbio.2019.12.011

Figure 3: Ciliary band neural progenitors project neurites and migrate small distances.

Figure 3:

(A) Whole mount in situ hybridization show that neural progenitors co-express ngn with brn1/2/4 in neural progenitors in the ciliary band. A single confocal section is shown. Side panels show multichannel and single channel images of highlighted regions. (B) Quantifications of cell counts done on double whole mount in situ hybridizations for ngn and brn1/2/4. A total of 68 embryos were assessed (embryos were either 26, 28, 30, or 32 hpf) and cells that expressed both genes or only ngn or brn1/2/4 were counted and scored as such. “n” represents the number of cells counted with the given condition. (C-E) Shows still images of embryos injected with Brn1/2/4 GFP BAC displaying neurites. Inset images show combined or split channel of the GFP expressing cell. Arrowhead indicates neurite projections. (F-F’) Shows still images from Supplementary Video 1 of an embryo injected with Brn1/2/4 GFP BAC. (F) Shows stills of combined DIC and GFP channels. (F’) Only the GFP fluorescent channel is shown. Time given in min:sec. Arrowhead indicates cell undergoing migration.