Clu particles are unique. (A) Structured illumination micrograph. Clu particles are granular and touch mitochondria in germ cells (inset), as previously shown. (B, C) Clu particles do not co-localize with autolysosomes (B) or Processing bodies (C) in germ cells. (D-G) Clu particles do not appear to associate with ER in germ cells (D), nor with ER-exit sites (E), cis-Golgi (F) or trans-Golgi (G) in surrounding somatic follicle cells. Green = ATP synthase (A), Atg8a (B), Tral::GFP (C), Sec61α::GFP (D), Sec23 (E), GMAP (F), and Golgin245 (G). Magenta = Clu (A-G). Scale bar: 5 μm in G for A-G, 2.5μm in A for inset.