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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 16;459(2):149–160. doi: 10.1016/j.ydbio.2019.12.004

Figure 3.

Figure 3.

Yeast Clu1p accumulates as cytoplasmic particles and associate with RpL3p. (A) A cartoon diagram of the shared domain structure between Drosophila Clu (Dm) and yeast Clu1p (Sc). (B) Serially diluted clu1Δ cultures grow normally on glucose, but not on glycerol. (C) clu1Δ forms small colonies after one week of growth on glycerol compared to its parental wild type strain. (D) Two independent isolates of clu1Δ. (BY4741 genetic background) form significantly higher percentages of petite colonies. (E) Representative micrographs showing petite colony formation. (F) Fixed and anti-GFP labeled log-phase yeast cells of strain clu1::CLU1-GFP. Clu1p puncta are indicated by arrows. (G) Co-immunoprecipitation shows that Clu1p associates with the ribosomal protein RpL3. Elongation of fatty acids protein 3 (Elo3p) serves as a negative control. Pulldowns were from clu1::CLU1-GFP extract. (H) The anti-GFP antibody is specific for clu1::CLU1-GFP. GFP pulldown from a wild type strain (W303) indicates there is no non-specific cross-reactivity. (I) Sucrose gradient using extract from yeast strain clu1::CLU1-GFP (top) and Drosophila wild type ovaries (bottom). Green = Clu1p::GFP, blue = DAPI. Scale bar: 5 μm in F for F. For D, p < 0.001 using a two-way ANOVA test.