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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 16;459(2):149–160. doi: 10.1016/j.ydbio.2019.12.004

Figure 4.

Figure 4.

Clu particles disaggregate in response to starvation. (A-C″). Follicles from w1118 females. Well-fed follicles contain many large particles in the germ cells (A′). Starvation for 5 hours on H20 causes the particles to disaggregate (B′). Re-feeding starved females causes Clu particles to reform (C′). Quantification is shown in (G). (D-F″) Surrounding somatic follicle cells show the same dynamic as the germ cells. ATP levels (H) and Clu levels (I, J) remain the same for all three conditions. clud08713 is a positive control. Details of n values are in the materials and methods. Error bars are S.E.M. Green = Clu (A-F″) and blue = DAPI (A-F″). Scale bar: 10 μm in C″ for A-C″ and in F″ for D-F″.