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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 16;459(2):149–160. doi: 10.1016/j.ydbio.2019.12.004

Figure 5.

Figure 5.

Clu particles and the Processing body component Trailer hitch respond oppositely to starvation. (A-C′) Follicles from Tral::GFP females. (A, C) Tral::GFP forms small aggregates in the nurse cells, homogeneous dispersed staining, and is concentrated at the anterior of the oocyte (A). Upon starvation, Tral::GFP forms very large processing bodies, the diffuse Tral signal decreases (A′, C′), whereas Clu particles disaggregate with Clu becoming diffuse (B′, C′). The dotted line indicates the oocyte (B). (E) Quantification of homogeneously dispersed Clu intensity in nurse cells under fed and starved conditions. (F) Quantification of Tral::GFP homogeneously dispersed intensity in nurse cells under fed and starved conditions. Details of n values are in the materials and methods. Green = Tral::GFP (A-A′, C-C′ ), magenta = Clu (B-B′, C-C′). Scale bar: 40μm in C′ for A-C′. Error bars are S.E.M. * p = 0.015, ** p = 0.0016 using a Student’s t-test with Welch’s correlation.