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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 16;459(2):149–160. doi: 10.1016/j.ydbio.2019.12.004

Figure 7.

Figure 7.

Oxidative stress disperses Clu particles. (A, B) Clu particles are always present in wild type siblings (n = 44) (A) but always missing in SOD2 mutant follicles (n = 51). (C, D) SOD2 mutants have decreased levels of ATP (C), but not decreased levels of Clu protein (D). (E-E″) Live-image stills of a well-fed cluCA06604 follicle. Addition of H202 causes particles to disperse (n = 11). Green = Clu (A, B), blue = DAPI (A, B), white = Clu::GFP (E-E″). Error bars are S.E.M. Scale bar: 10μm in B for A, B, 40μm in E′ for E-E′.