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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Dev Biol. 2019 Dec 16;459(2):149–160. doi: 10.1016/j.ydbio.2019.12.004

Figure 8.

Figure 8.

Stress causes mitochondrial mislocalization in Drosophila germ cells. (A-C) Follicles from w1118 females. Well-fed females have evenly dispersed mitochondria in germ cells (A). Starvation causes mitochondria to clump (arrow) (B). Refeeding yeast paste for two hours post-starvation causes mitochondria to disperse (C). (D, E) Mitochondria in SOD2 mutant germ cells also form clumps (E, arrow) compared to wild type SOD2/+ sibling follicles (D). This clumping is reminiscent of loss of Clu, as previously published (F). (G- G″) Stills from live-imaging well-fed cluCA06604. Adding H202 during imaging to initiate oxidative stress also causes mitochondria to clump. TMRE labeling of mitochondria indicates that mitochondria are initially dispersed at time zero (G), and that mitochondria start to clump after H202 addition (G′) (n = 6). At a later time-point, the TMRE labeling becomes spotty due to mitochondria losing their membrane potential and therefore their ability to sequester the dye (G″). Green = ATP synthase (A-F), blue = DAPI (A-F), white = TMRE (G-G″). Scale bars: 10 μm in C for A-C, G-G″, 10 μm in F for D-F.