(A) Read counts for the RNA-seq analysis. (B, C) Histograms, scatter
plots, and Spearman’s rank correlation coefficient comparing each
biological replicate of NF-YA DN with GFP (B) or PBCAB with GFP (C). (D) Venn
diagram showing upregulated genes (p-adj ≤ 0.01; FC ≥ 1.5) in
embryos injected with PBCAB or NF-YA DN. (E-I) GO terms associated with genes
upregulated (p-adj ≤ 0.01, FC ≥ 1.5) by PBCAB (E), upregulated by
NF-YA DN (F), upregulated by both PBCAB and NF-YA DN (G), downregulated
exclusively by PBCAB (H) or downregulated exclusively by NF-YA DN (I). In E-I,
blue bars correspond to transcription-related, green to embryogenesis-related,
orange to homeodomain-related, yellow to cilia-related, and gray bars to other
ontologies.