. Author manuscript; available in PMC: 2021 Mar 15.

Published in final edited form as: Dev Biol. 2019 Dec 17;459(2):161–180. doi: 10.1016/j.ydbio.2019.12.003

TABLE 1:

Primer sequences used to amplify putative enhancers from zebrafish genomic DNA.

Primer	Sequence^a
Tcf3a-enh1F1	ATGCCTCGAGTACTGCGTTAATCGCGCGTT
Tcf3a-enh1R1	ATGCCTCGAGGTTAGTGTGATATAATCTGT
Tle3a-enh1F1	ATGCCTCGAGGAAAAAAATAGATGACATTAC
Tle3a-enh1R1	ATGCCTCGAGGCTAGCGCTGGGAATACGA
Dachb-enh1F1	ATGCCTCGAGCGGTTTCTTTGCCATTCTTT
Dachb-enh1R1	ATGCCTCGAGAACTAAGAACAATGTACG
Fgf8a-XhoIenhF1	ATGCCTCGAGGGAGGTCGTTTGCGTATTTG
Fgf8a-XhoIenhR1	ATGCCTCGAGCTTGTCAATCCACCCTGCTT
Yap1-enhF1
Yap1-enhR1
Pax5-enh1F1	ATGCCTCGAGGCAAACGGATATTTTAAAAT
Pax5-enh1R1	ATGCCTCGAGGTGCGTAAAAATCCAAGTAA
Her6-enh1F1	ATGCCTCGAGTTCTTTTATAATTGTACTG
Her6-enh1R1	ATGCCTCGAGTGATGTAAATAGAAATACTG
Prdm14-XhoIenhF1	ATGCCTCGAGCCCCTCTTCTTTGTCCCTTG
Prdm14-XhoIenhR1	ATGCCTCGAGGGTAGGCTATCTGGACGGATAAT

Each primer contains an XhoI site (CTCGAG) to allow it to be cloned into the E1b-GFP-Tol2 vector with the exception of yap1, which contained BglII sites (AGATCT).