TABLE 1.
Strain | Gene | Primer pairs |
P. chrysosporium | LiP | F: 5′-TTCTTCGTCGAGACTCAG-3′ |
R: 5′-CTTGGACTGGTTGTTGAC-3′ | ||
P. chrysosporium | MnP | F: 5′-CTGTGAGTTACGGAATTGG-3′ |
R: 5′-GGAGGAGGAGGAAGTAGA-3′ | ||
P. chrysosporium | β-Actin | F: 5′-GCACCACACCTTCTACAA-3′ |
R: 5′-TCATCTTCTCACGGTTAGC-3′ | ||
L. edodes | LiP | F: 5′-GACGCCTTTCCCTGCTCTTACTG-3′ |
R: 5′-AGCAGCAGAACGAGCATATTCAGG-3′ | ||
L. edodes | MnP | F: 5′-CAATCCTCCGCTGTTACA-3′ |
R: 5′-TCGTTCGTGACCAAGATAA-3′ | ||
L. edodes | Lac | F: 5′-CCAACCATTCGTTCCTCTA-3′ |
R: 5′-GCAGTCAGCAATAGTAAGC-3′ | ||
L. edodes | β-Tublin | F: 5′-TTCACCGACAACATCACT-3′ |
R: 5′-GACATAACAAGGGACACAAG-3′ | ||
T. versicolor | LiP | F: 5′-CACGACCTTTCCATCTTC-3′ |
R: 5′-ATGCTTGCTGGTTAGTTG-3′ | ||
T. versicolor | MnP | F: 5′-GACACGCAGTTCTTCATC-3′ |
R: 5′-CTGGTTGTTGACGAAGGA-3′ | ||
T. versicolor | Lac | F: 5′-GCTATCCTCCGCTATGAT-3′ |
R: 5′-GCCGTTGATGAAGAAGTT-3′ | ||
T. versicolor | β-Actin | F: 5′-TATCCGTCGTGACCTCTA-3′ |
R: 5′-CGATCTTGACCTTCATACTTG-3′ |
All data were normalized to the internal control, and relative expression was calculated using the 2-DDCt method. The results were analyzed by one-way analysis of variance (ANOVA). Statistical analysis was performed using Statistical Product and Service Solutions (SPSS).