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. 2020 Mar 12;8:99. doi: 10.3389/fbioe.2020.00099

TABLE 1.

Primer pairs of the real-time PCR.

Strain Gene Primer pairs
P. chrysosporium LiP F: 5′-TTCTTCGTCGAGACTCAG-3′
R: 5′-CTTGGACTGGTTGTTGAC-3′
P. chrysosporium MnP F: 5′-CTGTGAGTTACGGAATTGG-3′
R: 5′-GGAGGAGGAGGAAGTAGA-3′
P. chrysosporium β-Actin F: 5′-GCACCACACCTTCTACAA-3′
R: 5′-TCATCTTCTCACGGTTAGC-3′
L. edodes LiP F: 5′-GACGCCTTTCCCTGCTCTTACTG-3′
R: 5′-AGCAGCAGAACGAGCATATTCAGG-3′
L. edodes MnP F: 5′-CAATCCTCCGCTGTTACA-3′
R: 5′-TCGTTCGTGACCAAGATAA-3′
L. edodes Lac F: 5′-CCAACCATTCGTTCCTCTA-3′
R: 5′-GCAGTCAGCAATAGTAAGC-3′
L. edodes β-Tublin F: 5′-TTCACCGACAACATCACT-3′
R: 5′-GACATAACAAGGGACACAAG-3′
T. versicolor LiP F: 5′-CACGACCTTTCCATCTTC-3′
R: 5′-ATGCTTGCTGGTTAGTTG-3′
T. versicolor MnP F: 5′-GACACGCAGTTCTTCATC-3′
R: 5′-CTGGTTGTTGACGAAGGA-3′
T. versicolor Lac F: 5′-GCTATCCTCCGCTATGAT-3′
R: 5′-GCCGTTGATGAAGAAGTT-3′
T. versicolor β-Actin F: 5′-TATCCGTCGTGACCTCTA-3′
R: 5′-CGATCTTGACCTTCATACTTG-3′

All data were normalized to the internal control, and relative expression was calculated using the 2-DDCt method. The results were analyzed by one-way analysis of variance (ANOVA). Statistical analysis was performed using Statistical Product and Service Solutions (SPSS).