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. 2020 Mar 18;10:4961. doi: 10.1038/s41598-020-61591-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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All previously known patterns occur in the top 40 genes when ranked according to their Transquartile Range (TQR). Expression summary plots of the top 40 genes, grouped by pattern, with an orange border indicating an unknown pattern. The arrangement of cells in each pattern is shown in the summary box on the right, together with the number of genes per pattern, whether known (black) or unknown (red). Genes whose patterns we validated are highlighted in orange. For each gene, the columns indicate the gene name, any previously known in situ pattern in blue (grey if not known), the average of the transformed expression values (clipped above 0.05), the pattern resulting from clustering, and finally, the TQR as the reliability score, scaled for visualization. The in situ probe for Tbx6-r.b also detects Tbx6-r.c and Tbx6-r.d (indicated by a black in situ pattern).