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. 2020 Mar 18;10:4961. doi: 10.1038/s41598-020-61591-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pattern discovery results are validated by in situ hybridisation and single-cell qPCR. (a–e) For each pattern being tested (left), the in situ hybridisation results are shown from the animal and vegetal side (middle). The arrowheads pointing to the expressing cells are shown on only one side of the embryo. Gene expression levels for each cell type were measured by single-cell qPCR. The qPCR measures for the cell types of each embryo were scaled to add to 1. The bar charts (right) show the mean of the resulting values of biological replicates, with whiskers indicating the range. The lines under the bars indicate the cells of the expression pattern being tested. The number of replicates of each gene is shown under each bar chart.