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. 2020 Mar 18;11(3):193. doi: 10.1038/s41419-020-2386-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Volcano plot showed genes that were differentially expressed after LV-AQP1 transfection. b Heatmap showed the changes in the expression of aging-related genes in aged and AQP1-overexpressing aged TSPCs. c–e Expressions of genes involved in tendon aging-related genes (MMP2, MMP9, DCN) were assessed by qRT-PCR in TSPCs. f β-gal staining for the senescent cells in TSPCs. Scale bars: 200 μm (larger images); 100 μm (inset). g Quantitative analysis of β-gal-positive TSPCs. h qRT-PCR for the cell cycle regulators (p16^INK4A, p21, p27, p53) levels in TSPCs. i Immunofluorescence staining for p16^INK4A in TSPCs. Scale bar: 50 μm. j Quantitative analysis of p16^INK4A-positive cells. k qRT-PCR for senescence-associated secretory phenotype (SASP) genes (MMP3, IL6, IL1B, IL1A and CXCL5) expressions in TSPCs. Values represent the mean ± SD. *P < 0.05, significantly different from the young or aged group.