Fig. 8. Deletion of CD98hc leads to reduced MHCII⁺ macrophages in competitive adoptive BM monocyte transfer experiments.

After isolation of bone marrow monocytes from B6 Ly5.1 (CD45.1⁺) and CD98hc^ΔCX3CR1 (CD45.2 YFP⁺), CD11b⁺ monocytes were injected into CCR2^−/− recipients. a Dot plots of bone marrow CD45.1 and CD45.2 monocytes mixtures before transfer into CCR2^−/− animals. CD11b⁺/Ly6C⁺ dot plots were obtained after gating either on CD45.1 or CD45.2 monocytes. b Dot plots were generated by gating on live CD11b⁺ colonic lamina propria monocytes and macrophages isolated from tamoxifen-treated CCR2^−/− recipients. c Ratios between CD45.2 YFP⁺ and CD45.1⁺ WT colonic lamina propria monocytes and macrophages isolated from corn oil- or tamoxifen-treated CCR2^−/− hosts 5 days after transfer normalized to input ratios. Each dot indicates one respective host. Experiment was performed once with four biological replicates in each group.