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. 2020 Mar 18;10:4921. doi: 10.1038/s41598-020-61307-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) ‘Search by miRNA’ output for miR-326-3p in the brainstem and spinal cord. Expression is readily detectable exclusively in microglia of the brainstem and astrocytes of the spine (n = 3–4 mice; mean ± SEM). (B) ‘Search by miRNA’ output for miR-326-3p in the cerebellum. miR-326-3p is depleted in Purkinje neurons, relative to the cerebellum as a whole (n = 2–3 mice; mean ± SEM). (C) ‘Search by miRNA’ output for miR-326-3p in the neocortex. miR-326-3p is readily detectable in cortical neurons (n = 2–3 mice; mean ± SEM). Only the more predominant and conserved 3p strand is displayed. The “Valencia” colored frame indicates that miR-326-3p is conserved among most mammals.