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. 2020 Mar 18;5:23. doi: 10.1038/s41541-020-0173-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Native PAGE of Pfs230C1 after 1 h incubation with indicated liposomes. The antigen:liposome mass ratio (on CoPoP or PoP basis) is indicated and 1.5 µg of Pfs230C1 was loaded in each lane. The absence of a protein band is indicative of antigen binding to the liposomes, which are too large to migrate in the gel. Representative of three independent experiments. b Binding of Pfs230C1 with varying amounts of CoPoP/PHAD or PoP/PHAD liposomes based on microcentrifugal filtration. c Pfs230C1 binding kinetics based on microcentrifugal filtration. d Size of liposomes before and after Pfs230C1 binding, based on dynamic light scattering. e Serum stability of CoPoP/PHAD with fluorophore-labeled Pfs230C1 during incubation for 2 weeks in 20% human serum at 37 ˚C. The asterisk shows the time after which 0.1% Triton X-100 and proteinase K were added. Graphs show mean ± std. dev. for n = 3 independent experiments. f Cryo EM image of Pfs230C1 with CoPoP/PHAD. Image was taken from a single experiment.