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. 2020 Mar 18;5:23. doi: 10.1038/s41541-020-0173-x

Fig. 2. Pfs230C1 adjuvanted with CoPoP/PHAD or ISA720, but not Alum, induces IgG with strong transmission-reducing activity.

Fig. 2

a, b Anti-Pfs230C1 IgG ELISA data (top) and standard membrane feeding assay functional assay (SMFA, bottom). a ICR mice were immunized with 1 μg of Pfs230C1 with indicated adjuvants. b ICR mice were immunized with micrograms of Pfs230C1 with Alum or nanograms of Pfs230C1 with CoPoP/PHAD or ISA720. ELISA experiments in (a, b) were performed with n = 10 independent mice; lines show geometric mean, while SMFA experiments were performed with n = 20 mosquitoes; lines show arithmetic mean. Black open circles in (a, b) represent control mice immunized with CoPoP/PHAD alone, without Pfs230C1. c Immunofluorescence assay of NF54 parasites at gametocyte stage. Images taken from a single experiment. Scale bar, 10 μm. For (a, b), log10-transformed titer were analyzed by one-way ANOVA test followed by Tukey’s comparisons. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For SMFA functional assay, the statistical differences were analyzed by a zero-inflated negative binomial model38.