Fig. 5. Mutant SF3B1 downregulates unproductive, intron-retaining transcripts.

a Schematic of productive and unproductive isoforms. The region on an isoform where stop codons can occur is colored red. Unproductive isoforms have premature stop codons present 55 nt or more from the last splice junction. b Expression fold-change (FC) between SF3B1^K700E and SF3B1^WT of FLAIR isoforms categorized as containing (IR) or not containing (spliced) retained introns and by productivity. The difference between the log2(FC) of the productive spliced and the unproductive-IR categories is 0.720. Violin plot show median as white dot, box limits are upper and lower quartile, and filled area represents the entire range of the kernel density estimation. *p value < 0.05, ***p value < 0.0005, two-sided Mann–Whitney U test. Source data are provided in a Source Data file.