Table 1.
Nanopore-sequencing statistics.
| Sample | Data (Gb) | Total reads | % Pass | % full length | Median pass read length | Mean pass read length | No. of genes detected | % genes with AS |
|---|---|---|---|---|---|---|---|---|
| Promethion WT 1 | 2.75 | 5,839,921 | 23.1 | 22.9 | 816 | 1020 | 17,369 | 53.0% |
| Promethion WT 2 | 0.526 | 2,051,438 | 6.91 | 27.7 | 768 | 936 | 8492 | 43.9% |
| Promethion WT 3 | 52.2 | 63,378,471 | 56.9 | 28.0 | 788 | 944 | 43,223 | 41.7% |
| Promethion MT 1 | 0.522 | 3,016,571 | 1.70 | 24.0 | 718 | 862 | 6272 | 31.8% |
| Promethion MT 2 | 91.1 | 93,130,856 | 74.8 | 30.2 | 870 | 1020 | 45,744 | 41.2% |
| Promethion MT 3 | 3.1 | 15,101,288 | 5.38 | 30.2 | 919 | 1123 | 16,218 | 54.7% |
| Promethion B cell 1 | 65.8 | 66,311,147 | 60.7 | 34.3 | 948 | 1099 | 33,538 | 46.6% |
| Promethion B cell 2 | 0.970 | 5,390,853 | 7.16 | 33.5 | 712 | 951.3 | 13,638 | 44.0% |
| Promethion B cell 3 | 0.731 | 2,934,130 | 4.80 | 27.8 | 766 | 1015 | 9372 | 34.6% |
| Minion WT 1 | 0.105 | 119,091 | – | 19.6 | 651 | 868 | 8320 | 36.6% |
| Minion MT 1 | 0.138 | 129,798 | – | 25.5 | 880 | 1084 | 9747 | 42.6% |
| Minion B cell 1 | 0.042 | 46,077 | – | 32.5 | 749 | 922 | 5379 | 26.8% |
For each run, the following statistics were computed: gigabase of data, total reads, the percentage of reads designated as passing by the basecaller for PromethION runs, percentage of aligning pass reads that are full-length, median pass read length, mean pass read length, the number of genes covered, and the number of genes with evidence of alternative splicing (“Methods”). For the 2D MinION runs, the total number of reads is the number of 2D reads, which was also considered the total number of pass reads.