Fig. 2. Connexin channel inhibitors reduce γ-H2AX responses in the irradiated and bystander areas.
a Gamma-H2AX staining in RBE4 cells irradiated with the 3 × 3 mm collimator, treated with vehicle (left) or Gap26 (right; scale bars 1 mm). b Effect of connexin channel inhibition on γ-H2AX scores (normalized to vehicle) in RBE4 cells in the irradiated zone (n = 8–9). c Effect of connexin channel inhibition on bystander γ-H2AX (normalized to vehicle) in RBE4 (n = 8–9). d, e Experiments as in b and c but performed on pBMECs (n = 4–10). f γ-H2AX scores in the bystander area in pBMECs from C57BL/6 Cx43fl/fl:Tie2-Cre mice (n = 12); western blotting analysis (left) demonstrating decreased Cx43 expression in C57BL/6 Cx43fl/fl:Tie2-Cre.