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. 2020 Mar 12;11:436. doi: 10.3389/fimmu.2020.00436

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Copyright © 2020 Huang, Shi, Hu, Wu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microbial polysaccharide-binding activity was investigated by ELISA. LPS from E. coli or PGN from M. luteus was used to coat plates. rToCTL1-CRD (A) and rToCTL2-CRD (B) were diluted into different concentrations (0.78, 1.56, 3.125, 6.25, 12.5, and 25 μg/mL) and added to the polysaccharide-coated plates. After incubation with anti-GST antiserum, the interaction was detected with anti-rabbit IgG secondary antibody at 450 nm. The control wells were filled with rGST protein. Results were obtained based on three independent repeats.