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. 2020 Mar 12;11:436. doi: 10.3389/fimmu.2020.00436

Figure 7.

Figure 7

Recombinant expression and purification analysis of rToCTL1-CRD (A) and rToCTL1-CRD (B). Lane M, markers; Lane 1, the bacteria liquid before IPTG induction; Lane 2, the pGEX-6p-2-rToCTL1-CRD or pGEX-6p-2-rToCTL2-CRD was induced with 0.5 mM IPTG at 28°C for 6 h; Lane 3, purified rToCTL1-CRD or rToCTL2-CRD fusion protein.