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. 2020 Mar 12;11:436. doi: 10.3389/fimmu.2020.00436

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Copyright © 2020 Huang, Shi, Hu, Wu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding activity of rToCTL1-CRD and rToCTL2-CRD to microorganisms. Seven kinds of bacteria (G+ bacteria S. aureus, M. luteus, B. subtilis, B. thuringiensis, and G– bacteria V. parahaemolyticus, V. harveyi, A. hydrophila) were incubated with rToCTL1-CRD or rToCTL2-CRD and then washed three times with TBS and subjected to elution with 5% SDS. The binding activity was confirmed by Western blotting analysis. rToCTL1-CRD and rToCTL2-CRD were detected by monoclonal antibody against GST. The binding activity of GST protein was tested and used as the negative control.