Fig. 2. ALDOA determines the cancer properties and self-renewal capability of lung cancer.
a Representative images of the density and morphology of spheroids at various magnifications using a 2nd population of the vector control and ALDOA expression construct in CL1-0 cells. b Quantitative analysis results of the spheroid numbers and diameters after control and ALDOA overexpression treatment are shown in the panels. c The qRT-PCR results show the mRNA expression of POU5F1, SOX2, NANOG, CD133 and Nestin with or without overexpression of an exogenous ALDOA gene in CL1-0 cells. d Western blot analysis showing the protein levels of ALDH1A3, Oct4, ALDOA, Nanog, CD44 and BTK with or without overexpression of an exogenous ALDOA-encoding gene in CL1-0 monolayer and sphere-forming cells. e, f An overview of the appearance of mice implanted with ALDOA-overexpressing CL1-0 sphere-forming cells by a subcutaneous injection in the right flank using serially diluted cells. In c, GAPDH was used as an internal loading control. The significance of the differences in b and c was analyzed using Student’s t-test.