TABLE 1.
Comparison of HPLC, GC-MS, and LC-MS/MS analysis for the dendrobine peak retention times and its molecular weight.
Sample type | Dendrobine peak retention time (min) | ||
HPLC | GC-MS | LC-MS/MS | |
5.30 ± 0.02 | 14.97 ± 0.02 | 7.51 ± 0.02 | |
Trichoderma longibrachiatum MD33 | 5.28 ± 0.02 | 14.96 ± 0.02 | 7.51 ± 0.02 |
Chemical reference standard dendrobine | 5.30 ± 0.02 | 14.96 ± 0.02 | 7.57 ± 0.02 |
Wild Dendrobium nobile stem dendrobine | 5.32 ± 0.02 | 14.97 ± 0.02 | 7.48 ± 0.02 |
Dendrobine molecular weight | NA | 263 | 264.195 |
All the values are the standard deviation of three replicates; NA: not available.