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. 2020 Mar 12;10:280. doi: 10.3389/fonc.2020.00280

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Copyright © 2020 Tang, Chen, Chen, Jiang, Yan, Mo, Tang and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DCST1-AS1 interacts with ANXA1 in BT-549 cells. (A) Gel electrophoresis of the PCR products of the sense and antisense strands of DCST1-AS1. (B) SDS-PAGE of proteins pulled down using the DCST1-AS1 sense and antisense strands. (C) Analysis of the pulled-down proteins by liquid chromatography/tandem mass spectrometry (D) Western blotting was used to confirm the results of mass spectrometry. ANXA1 was detected in the proteins pulled down with the DCST1-AS1 sense strand. Proteins pulled down with the DCST1-AS1 antisense strand were used as a negative control, and whole-cell lysate was used as a positive control. (E) The RNA immunoprecipitation products were isolated and purified, and the amount of DCST1-AS1 bound to ANXA1 or IgG was measured by RT-qPCR. IgG was used as a negative control. 18S rRNA was used as an internal control. **p < 0.01.