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. 2020 Mar 19;15:62. doi: 10.1186/s11671-020-3295-1

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a) FTIR spectra of Au@SiO₂ nanoparticles, b) size distribution of FA-MTX conjugated Au@SiO₂ NPs measured by dynamic light scattering (DLS) c) The zeta potential of Au@SiO₂ and FA-MTX conjugated Au@SiO₂ NPs measured by dynamic light scattering (DLS) at pH=7.4 and T=25 °C, d) Chromatogram of unloaded MTX and FA separated from FA-MTX conjugated Au@SiO₂ NP measured simultaneously by HPLC method