Table 2.
Induction expression conditions of FlaC strain
| No. | A | B (mmol/L) | C (h) | D (℃) | Optical Density/SD (×104) |
|---|---|---|---|---|---|
| 1 | 0.5 | 0.1 | 3 | 28 | 4.708 ± 0.393 |
| 2 | 0.5 | 0.3 | 8 | 32 | 5.957 ± 0.242 |
| 3 | 0.5 | 0.5 | 12 | 37 | 7.310 ± 0.070 |
| 4 | 0.8 | 0.1 | 8 | 37 | 7.534 ± 0.299 |
| 5 | 0.8 | 0.3 | 12 | 28 | 6.311 ± 0.579 |
| 6 | 0.8 | 0.5 | 3 | 32 | 5.220 ± 0.215 |
| 7 | 1.0 | 0.1 | 12 | 32 | 5.575 ± 0.292 |
| 8 | 1.0 | 0.3 | 3 | 37 | 5.726 ± 0.241 |
| 9 | 1.0 | 0.5 | 8 | 28 | 4.016 ± 0.260 |
| K1 (mean value 1) | 5.992 | 5.999 | 5.518 | 6.012 | |
| K2 (mean value 2) | 6.355 | 5.998 | 6.527 | 5.584 | |
| K3 (mean value 3) | 5.107 | 5.515 | 6.399 | 5.859 | |
| Range analysis | 1.249 | 0.484 | 1.009 | 0.428 | |
A, B, C, and D mean the induction strain OD600 value, induction of IPTG concentration, induction time, and induction temperature, respectively. Compared to the analysis size of the mean value of K1, K2, and K3, the optimal inducing expression condition of FlaC protein was A2, B1, C2, and D1, respectively.