Figure 1.

Experimental flowchart of PMS model establishment. Time points of the body weight measurements (circles), the open field test (hexagon), the vaginal smear test (triangles), and the sample collection (double triangles) are shown. Vaginal smear method was used to select the rats with regular estrous cycles (a cycle for 4 to 5 days). Then, these rats were weighed and subjected to open field test. After that, they were grouped. Each group contained both rats in the reception phase and nonreception phase. In the following estrus cycle, PMS modeling was carried out and corresponding drugs were given. After modeling, samples were collected.