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MSCs spontaneously form spheroids in TeSR-E8 medium on tissue culture dishes. (a) Phase contrast images showing the time-course of ADSC spheroid formation over 4 days. Scale bar: 150 μm. (b) Size distribution of ADSC spheroids on day 4 postculture. (c, d) Morphology of monolayer and spheroid BMSCs and DPSCs. Scale bar: 150 μm.
