FIGURE 4.

L-Kynurenine-primed cDCs promote in vitro induction of LAP⁺ T cells. Representative cytofluorimetric dot plots and relative analysis of percent expression of LAP (A) and TNF-α (B) coexpression in CD4⁺ cells co-cultured for 96 h with cDCs either unprimed or LPS-primed and then incubated for 24 h with Kyn at 50 μM. A mouse IgG2a antibody was used as isotype control. Shown in upper right quadrants are percentages of double-positive cells. (C) IL-10 production was measured in cells treated as in (A). Data are means ± SD of three independent experiments. *P < 0.05, **P < 0.01 (two-way ANOVA).