Fig. 2.

QRT-PCR analyzes of genes involved in cell migration. VEGF (A), CXCR-4 (B), VLA-4 (C), VCAM-1 (D) and MMP-2 (E) mRNA expression in hUMSCs after treatment (2D, 4D, 6D) with MPLA and SLA. The graphs show that both treatments led to an increase in expression level of VEGF at all studied times. The data show that under the influence of MPLA, CXCR-4 mRNA expression increased on the fourth day (P = 0.009), and after treatment with SLA, a slight decrease was identified on the fourth day (P = 0.004) as compared with the control. Also, under the influence of MPLA and SLA, VLA-4 mRNA expression increased and decreased on days 2, 4, 6 (P < 0.05), respectively. VCAM-1 mRNA expression increased at all of studied times (P < 0.05) in comparison with the control. MMP-2 mRNA expression was up-regulated in hUMSCs treated with MPLA and SLA at all studied times. Data were normalized to the expression levels of GAPDH in the control. 2 day, 4 day, 6 day. All values are expressed as mean ± SD for 6 days in each group; * MPLA vs control, $ SLA vs control and # MPLA vs SLA