Table 3.
Effect of BPA and NG on enzymatic and non-enzymatic antioxidants
|
Gropups
variables |
Control | BPA 50 mg/kg | BPA 50 mg/kg+NG 40 mg/kg | BPA 50 mg/kg+NG 80 mg/kg | BPA 50 mg/kg+NG 160 mg/kg | NG 160 mg/kg |
|---|---|---|---|---|---|---|
| MDA (nmol/mg protein) | 1.16 ± 0.02 | 2.89 ± 0.04*** | 2.78 ± 0.06 | 2.80 ± 0.05 | 2.61 ± 0.04# | 1.2±0.81 |
| ROS (% of control) | 100 ±10.1 | 197.87±12.61*** | 166.65±11.76 | 141.76±11,6## | 117.64±14.7### | 94.89±17.1 |
| GSH (nmol/mg protein) | 100 ±2.6 | 25.48 ±5.2*** | 24.5 ±5.8 | 50.25 ±4.8## | 72.1 ±3.2### | 107.27 ±5.4** |
| Catalase (µmol/ mgprotein) | 50.22±1.34 | 22.48±3.71*** | 22.3±5.8 | 29.7±2.9# | 34.80±8.62## | 51.98±1.87 |
| SOD (U/mg protein) | 1.7±0.26 | 1.1±0.49** | 1.14±0.49 | 1.22±0.59 | 1.32±0.48# | 1.77±0.79 |
| GSH-Px (µmol/mg protein) | 1.3±0.47 | 0.9±0.17* | 0.9±0.05 | 1.03±0.07 | 1.14±0.45# | 1.35±0.99 |
Data are mean SD; n = 8. Difference between control and other groups is significant at P < 0.001(***), P < 0.01 (**) and P < 0.05 (*). Difference between BPA-treated and other groups is significant at P < 0.001(###), P < 0.01 (##) and P < 0.05 (#). BPA: bisphenol A; NG: naringin; MDA: malondialdehyde; ROS: reactive oxygen species; GSH: glutathione; SOD: superoxide dismutase; CAT: catalase; GSH-Px: glutathione peroxidase. P values were from one-way ANOVA, followed by Tukey’s test for multiple comparisons.