Fig.4.

Relationship between EDN1 and VEGF in brain tissue homogenates. Relationship between EDN1 and VEGF in left middle (LM) and right middle (RM; infusion site) homogenates. Tissue concentrations of VEGF versus EDN1 in animals given Zibotentan (+zib; n = 36) in (a) RM and (b) LM and those without Zibotentan (-zib; n = 23) for (c) RM and (d) LM samples, showing best-fit (solid) regression line + 95% CI (dotted) in all animals. There was a significant positive correlation between VEGF and EDN1 in both (a) RM and (b) LM homogenates on analysis of those animals not given Zibotentan (RM r² = 0.470, p = 0.0038; LM r² = 0.128, p = 0.0320) but there was no correlation between VEGF and EDN1 in either cerebral hemisphere in the Zibotentan-treated animals (RM r² = 0.0168, p = 0.556; LM r² = 0.0131, p = 0.604).